{"id":3119,"date":"2015-03-05T16:10:03","date_gmt":"2015-03-05T08:10:03","guid":{"rendered":"http:\/\/wp.kmu.edu.tw\/rd\/?p=3119"},"modified":"2015-03-05T16:10:03","modified_gmt":"2015-03-05T08:10:03","slug":"%e7%a0%94%e7%a9%b6%e6%96%b0%e7%9f%a5-27","status":"publish","type":"post","link":"https:\/\/wp.kmu.edu.tw\/rd\/archives\/3119","title":{"rendered":"\u7814\u7a76\u65b0\u77e5"},"content":{"rendered":"<p style=\"text-align: right\"><a style=\"font-style: normal;text-align: right\" href=\"http:\/\/wp.kmu.edu.tw\/rd\/archives\/3092\">\u56de\u7b2c0030\u671f\u4e3b\u9801<\/a><\/p>\n<h1><span style=\"font-size: x-large;color: #c10000\">\u4e00\u3001\u7814\u7a76\u65b0\u77e5<\/span><\/h1>\n<p><a name=\"ResNew1\"><\/a><\/p>\n<h1><span style=\"color: #0000ff\"><span style=\"color: #0000ff\">Minipool caprylic acid fractionation of plasma using disposable equipment: a practical method to enhance immunoglobulin supply in developing countries<br \/>\n<\/span><\/span><\/h1>\n<p>Magdy El-Ekiaby, Mari\u00e1ngela Vargas, Makram Sayed, George Gorgy, Hadi Goubran, Mirjana Radosevic, Thierry Burnouf<\/p>\n<p>PLOS Neglected Tropical Diseases, 2015; 9 (2): e0003501, DOI: 10.1371\/journal.pntd.0003501<\/p>\n<p>\u4f0a\u6ce2\u62c9\u75c5\u6bd2\u8086\u8650\u975e\u6d32\uff0c\u9020\u6210\u8cf4\u6bd4\u745e\u4e9e\u3001\u7345\u5b50\u5c71\u5171\u548c\u570b\u7b49\u897f\u975e\u570b\u5bb6\u8fd1\u842c\u4eba\u6b7b\u4ea1\uff0c\u76ee\u524d\u96d6\u6709\u514d\u75ab\u7403\u86cb\u767d\u7528\u65bc\u6cbb\u7642\uff0c\u4f46\u7814\u767c\u904e\u7a0b\u7e41\u8907\u4e14\u6210\u672c\u9ad8\u3002\u514d\u75ab\u7403\u86cb\u767dG\uff08IgG\uff09\u662f\u4e16\u885b\u7d44\u7e54\u6297\u75c5\u6bd2\u57fa\u672c\u85e5\u7269\uff0c\u4f46\u4e5f\u662f\u767c\u5c55\u4e2d\u570b\u5bb6\u7f3a\u4e4f\u7684\u91cd\u8981\u8840\u6f3f\u884d\u751f\u7684\u85e5\u7269\u3002\u767c\u5c55\u4e2d\u570b\u5bb6\u9762\u81e8\u6cbb\u7642\u7269\u8cea\u56b4\u91cd\u77ed\u7f3a\uff0cIgG\u7684\u77ed\u7f3a\u8b93\u514d\u75ab\u7f3a\u9677\u7684\u60a3\u8005\u7121\u6cd5\u63a5\u53d7\u6cbb\u7642\uff0c\u4e26\u4e14\u66b4\u9732\u5728\u7576\u5730\u75c5\u539f\u9ad4\u53cd\u8986\u611f\u67d3\u3002\u6cd5\u570b\u79d1\u5b78\u5bb6Thierry Burnouf\u9577\u671f\u81f4\u529b\u65bc\u8840\u6db2\u88fd\u54c1\u5de5\u7a0b\u53ca\u75c5\u6bd2\u5b89\u5168\u6027\u7814\u7a76\uff0c\u4ed6\u6307\u51fa\uff0c\u4e00\u822c\u88fd\u5099\u8840\u6f3f\u514d\u75ab\u7403\u86cb\u767d\uff0c\u9700\u8981\u7e41\u8907\u6b65\u9a5f\u3001\u9ad8\u6210\u672c\u53ca\u88fd\u85e5\u7b49\u7d1a\u8a2d\u5099\uff0c\u5728\u7814\u7a76\u5718\u968a\u52aa\u529b\u4e0b\uff0c\u7814\u767c\u51fa\u7c21\u55ae\u65b9\u5f0f\uff0c\u9019\u500b\u65b9\u5f0f\u662f\u7a2e\u5c01\u9589\u5f0f\u4e14\u4e00\u6b21\u6027\u4f7f\u7528\u91ab\u7642\u8a2d\u5099\u3002Thierry Burnouf\u8207\u57c3\u53ca\u91ab\u9662\u7814\u7a76\u5718\u968a\uff0c\u767c\u73fe\u53ea\u8981\u5728\u5c01\u9589\u5f0f\u7684\u5851\u81a0\u88fd\u8840\u888b\uff0c\u52a0\u5165\u5316\u5b78\u8a66\u5291\u300c\u8f9b\u9178\u300d\uff0c\u7d93\u904e\u5145\u5206\u6416\u66431\u5c0f\u6642\uff0c\u518d\u96e2\u5fc3\u53d6\u51fa\u4e0a\u6e05\u6db2\uff0c\u5229\u7528\u900f\u6790\u79fb\u9664\u8f9b\u9178\u3001\u6fc3\u7e2e\u548c\u904e\u6ffe\u7b49\u6b65\u9a5f\uff0c\u5373\u53ef\u88fd\u6210\u514d\u75ab\u7403\u86cb\u767d\uff0c\u6210\u679c\u5df2\u520a\u767b\u65bcPLOS Neglected Tropical Diseases -Minipool Caprylic Acid Fractionation of Plasma Using Disposable Equipment: A Practical Method to Enhance Immunoglobulin Supply in Developing Countries\u3002\u5c0d\u65bc\u5c0d\u6297\u985e\u4f3c\u4f0a\u6ce2\u62c9\u75c5\u6bd2\u7684\u75ab\u75c5\u50b3\u67d3\uff0c\u53ea\u8981\u5f9e\u611f\u67d3\u800c\u50e5\u5016\u5b58\u6d3b\u4e0b\u4f86\u8005\u8eab\u4e0a\uff0c\u53d6\u5f97\u8840\u6f3f\u88fd\u6210\u514d\u75ab\u7403\u86cb\u767d\uff0c\u8f38\u6ce8\u5230\u611f\u67d3\u8005\u9ad4\u5167\u5c31\u80fd\u5c0d\u6297\u75c5\u6bd2\uff0c\u9019\u5957\u7cfb\u7d71\u5df2\u7372\u5f97\u975e\u6d32\u6148\u5584\u6a5f\u69cbGlobal Emerging Pathogen(GEP)\u9752\u775e\u548c\u975e\u6d32\u8840\u6db2\u4e2d\u5fc3\u540c\u610f\uff0cGPE\u7372\u6bd4\u723e\u84cb\u8332\u57fa\u91d1\u6703\u8d0a\u52a9\uff0c\u6c7a\u5b9a\u63a1\u7528\u9019\u5957\u88fd\u5099\u514d\u75ab\u7403\u86cb\u767d\u7684\u65b9\u6cd5\uff0c\u4f5c\u70ba\u4e0b\u6b21\u4f0a\u6ce2\u62c9\u75c5\u6bd2\u4f86\u8972\u6642\uff0c\u964d\u4f4e\u50b7\u4ea1\u7684\u7dca\u6025\u6551\u63f4\u63aa\u65bd\u3002\u5168\u6587\u8acb\u9023\u7d50http:\/\/journals.plos.org\/plosntds\/article?id=10.1371\/journal.pntd.0003501\u8207\u76f8\u95dc\u806f\u7d50http:\/\/www.sciencedaily.com\/releases\/2015\/02\/150226144901.htm\u3002<br \/>\n\u82f1\u6587\u6458\u8981<br \/>\nPlasma-derived immunoglobulin G (IgG) is on WHO\u2019s Essential Medicines List, yet developing countries face severe shortages of this critical treatment. Infusion of IgG prepared from locally-collected plasma provides an advantageous mix of antibodies to viral and bacterial pathogens found in the living environment, and this can reduce recurrent infections in immune-deficient patients. We developed a simple manufacturing process using disposable equipment (blood bags, hemodialyzer, and filters) to isolate immunoglobulins from minipools of 20 plasma donations. This process yields a ca. 90% pure virally-inactivated immunoglobulin fraction at 50\u201360% recovery. Anti-hepatitis B and anti-rubella immunoglobulins were enriched fourfold to sixfold. The product was free of in-vitro thrombogenic and proteolytic activity, confirming its expected clinical safety profile. Virus validations showed caprylic acid treatment robustly inactivated or removed infectivity of lipid-enveloped viruses, including human immunodeficiency virus (HIV) and hepatitis C virus model. This simple and cost-effective process is implemented in Egypt to prepare experimental batches for clinical evaluation. It can enhance immunoglobulin supplies to treat immunodeficient patients through passive transmission of antibodies directed against local pathogens. The method requires minimal training and reasonable infrastructure, and is a practical means to prepare convalescent hyperimmune IgG during infectious outbreaks such as the current Ebola episode.<\/p>\n<p style=\"text-align: right\"><a href=\"http:\/\/wp.kmu.edu.tw\/rd\/archives\/3092\">\u56de\u7b2c0030\u671f\u4e3b\u9801<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u56de\u7b2c0030\u671f\u4e3b\u9801 \u4e00\u3001\u7814\u7a76\u65b0\u77e5 Minipool caprylic acid  &hellip; <a href=\"https:\/\/wp.kmu.edu.tw\/rd\/archives\/3119\">\u95b1\u8b80\u5168\u6587 <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[38],"tags":[],"class_list":["post-3119","post","type-post","status-publish","format-standard","hentry","category-38"],"_links":{"self":[{"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/posts\/3119","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/comments?post=3119"}],"version-history":[{"count":1,"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/posts\/3119\/revisions"}],"predecessor-version":[{"id":3120,"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/posts\/3119\/revisions\/3120"}],"wp:attachment":[{"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/media?parent=3119"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/categories?post=3119"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wp.kmu.edu.tw\/rd\/wp-json\/wp\/v2\/tags?post=3119"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}