{"id":2,"date":"2013-03-12T12:47:38","date_gmt":"2013-03-12T04:47:38","guid":{"rendered":"http:\/\/wp.kmu.edu.tw\/tzupinw\/?page_id=2"},"modified":"2025-12-16T11:51:21","modified_gmt":"2025-12-16T03:51:21","slug":"sample-page","status":"publish","type":"page","link":"https:\/\/wp.kmu.edu.tw\/tzupinw\/","title":{"rendered":"\u738b\u5b50\u658c \u526f\u6559\u6388 (Tzu-Pin Wang, Ph.D., Associate Professor)"},"content":{"rendered":"\n\n\n
\u00a0<\/strong>Here for English<\/strong><\/span><\/a><\/td>\n\u00a0<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"image002\"<\/a><\/p>\n

\u8fa6\u516c\u5ba4\uff1a<\/b>N817\u5be6\u9a57\u5ba4(N835\u5f8c\u9762)<\/p>\n

\u7814\u7a76\u5ba4\uff1a<\/b>N835\u751f\u7269\u89f8\u5a92\u5be6\u9a57\u5ba4
\n\u96fb\u3000\u8a71\uff1a<\/b>073121101 ext 2756
\nE-mail<\/b>\uff1a<\/b>tzupinw@kmu.edu.tw<\/a><\/p>\n

 <\/p>\n

\u5b78\u6b77\uff1a<\/b>
\n\u570b\u7acb\u53f0\u7063\u5927\u5b78\u68ee\u6797\u7cfb\u5b78\u58eb(1989)
\n\u7f8e\u570b\u52a0\u5dde\u5dde\u7acb\u5927\u5b78Fullerton\u5206\u6821\u5316\u5b78\u66a8\u751f\u7269\u5316\u5b78\u7cfb\u78a9\u58eb(1994)
\n\u7f8e\u570b\u7d10\u7d04\u5dde\u5dde\u7acb\u5927\u5b78\u4ee5\u53caSyracuse\u5927\u5b78\u5316\u5b78\u7cfb\u535a\u58eb(2000)<\/p>\n

 <\/p>\n

\u7d93\u6b77\uff1a<\/b><\/p>\n

\u7f8e\u570b\u7d10\u7d04\u5dde\u7acb\u5927\u5b78\u6c34\u725b\u57ce\u5206\u6821(State University of New York, University at Buffalo)\u751f\u7269\u5316\u5b78\u7cfb\u535a\u58eb\u5f8c\u7814\u7a76\u54e1(2000.12 – 2002.12)
\n\u7f8e\u570b\u7d10\u7d04\u5dde\u7acb\u5927\u5b78\u6c34\u725b\u57ce\u5206\u6821(State University of New York, University at Buffalo)\u5316\u5b78\u7cfb\u535a\u58eb\u5f8c\u7814\u7a76\u54e1(2003.1 – 2005.8)
\n\u9ad8\u96c4\u91ab\u5b78\u5927\u5b78\u91ab\u85e5\u66a8\u61c9\u7528\u5316\u5b78\u7cfb\u52a9\u7406\u6559\u6388(2005.9 \u2013 2013.2)<\/p>\n

\u9ad8\u96c4\u91ab\u5b78\u5927\u5b78\u91ab\u85e5\u66a8\u61c9\u7528\u5316\u5b78\u7cfb\u526f\u6559\u6388(2013.2 \u2013 \u8fc4\u4eca)<\/p>\n

 <\/p>\n

\u738b\u5b50\u658c\u526f\u6559\u6388\u2500\u751f\u7269\u89f8\u5a92\u5316\u5b78\u5be6\u9a57\u5ba4\u7c21\u8ff0<\/b><\/p>\n

\u672c\u5be6\u9a57\u5ba4\u7814\u7a76\u5145\u5206\u7d50\u5408\u50b3\u7d71\u6709\u6a5f\u5408\u6210\u5316\u5b78\u3001\u5316\u5b78\u52d5\u529b\u5b78\u3001\u7121\u6a5f\u5316\u5b78\u3001\u751f\u7269\u5316\u5b78\u3001\u53ca\u5206\u5b50\u751f\u7269\u6280\u8853\uff0c\u914d\u5408\u5728\u5206\u6790\u5316\u5b78\u53ca\u6750\u6599\u5316\u5b78\u7684\u61c9\u7528\uff0c\u65bc\u57fa\u790e\u7684\u5316\u5b78\u539f\u7406\u63a2\u8a0e\u4e0b\uff0c\u6709\u56db\u5927\u7814\u7a76\u65b9\u5411\uff1a\u6838\u9178\u9176(ribozyme)\u3001\u6838\u9178\u85e5\u7269\u3001\u5316\u5b78\u63a2\u91dd(chemical probes)\u3001\u74ca\u916f\u91a3(agarose)\u3002\u672c\u5be6\u9a57\u5ba4\u76ee\u524d\u7b2c\u4e00\u500b\u7814\u7a76\u91cd\u9ede\uff0c\u5728\u65bc\u7814\u7a76\u548c\u9178\u5206\u5b50\u7d50\u69cb\u53ca\u5176\u751f\u7269\u6280\u8853\u958b\u767c\u3002\u6211\u5011\u7684\u7b2c\u4e8c\u500b\u7814\u7a76\u91cd\u9ede\uff0c\u4fc2\u6839\u64da\u672c\u5be6\u9a57\u5ba4\u5148\u524d\u767c\u5c55\u51fa\u7684\u6838\u9178phosphormaidation\u53cd\u61c9\uff0c\u61c9\u7528\u65bc\u66f4\u6709\u6548\u7684\u5408\u6210\u6838\u9178\u7d50\u69cb\u4fee\u98fe\u6a19\u5b9a\u9375\u7d50\u7522\u7269\u53capeptide-oligonucleotide conjugates (POCs)\u65b9\u6cd5\u7684\u958b\u767c\uff0c\u7d50\u5408bioorthogonal\u53cd\u61c9\uff0c\u767c\u5c55\u9ad8\u6548\u80fd\u6838\u9178\u5316\u5b78conjugation\u65b9\u6cd5\u53ca\u61c9\u7528\uff0c\u671f\u8a31\u672a\u4f86\u80fd\u767c\u5c55\u51fa\u66f4\u5177\u5c08\u4e00\u6027\u4e14\u7121\u526f\u4f5c\u7528\u7684\u6838\u9178\u85e5\u7269\uff0c\u9054\u5230\u75be\u75c5\u6cbb\u7642\u7684\u76ee\u6a19\u3002\u672c\u5be6\u9a57\u5ba4\u7b2c\u4e09\u500b\u7814\u7a76\u91cd\u9ede\uff0c\u70ba\u6709\u6a5f\u5408\u6210\u5316\u5b78\u63a2\u91dd\uff0c\u53ef\u4f5c\u70ba\u5728\u6a23\u54c1\u6216\u751f\u7269\u9ad4\u5167\uff0c\u5373\u6642\u7684\u87a2\u5149\u5b9a\u91cf\u8207\u986f\u5f71\u5206\u6790\uff0c\u4f5c\u70ba\u9175\u7d20\u6d3b\u6027\u6aa2\u6e2c\u6216\u65e9\u671f\u75be\u75c5\u767c\u751f\u4e4b\u8a66\u5291\u3002\u672c\u5be6\u9a57\u5ba4\u76ee\u524d\u7b2c\u56db\u500b\u7814\u7a76\u91cd\u9ede\uff0c\u662f\u7d93\u7531\u5c0d\u74ca\u916f\u91a3\u7684\u57fa\u790e\u7269\u6027\u53ca\u5316\u5b78\u7d50\u69cb\u4e4b\u63a2\u8a0e\uff0c\u7531\u53f0\u7063\u672c\u571f\u7d05\u85fb\u985e\u7d14\u5316\u51fa\u751f\u91ab\u5206\u751f\u7814\u7a76\u7b49\u7d1a\u7684\u9ad8\u7d14\u5ea6\u4f4e\u80cc\u666f\u503c\u7684\u74ca\u916f\u91a3\uff0c\u53ca\u5176\u884d\u751f\u7269\u4e4b\u958b\u767c\u3002\u672a\u4f86\u5be6\u9a57\u5ba4\u4e5f\u5e0c\u671b\u80fd\u9032\u884c\uff0c\u95dc\u65bc\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u7684\u57fa\u790e\u751f\u7269\u7121\u6a5f\u5316\u5b78\u53ca\u9175\u7d20\u52d5\u529b\u5b78\u539f\u7406\u548c\u751f\u5316\u5206\u751f\u6280\u8853\u7684\u8a13\u7df4\u5916\uff0c\u53e6\u5916\u5728\u6709\u6a5f\u5408\u6210\u65b9\u6cd5\u53ca\u5176\u4ed6\u57fa\u790e\u5316\u5b78\u9818\u57df\u4e0a\u7372\u5f97\u5145\u5be6\u5b8c\u6574\u7684\u7814\u7a76\u80fd\u529b\uff0c\u5c0d\u65bc\u5b78\u751f\u672a\u4f86\u5f9e\u4e8b\u8de8\u9818\u57df\u5b78\u7fd2\u6703\u7814\u7a76\uff0c\u5c31\u6703\u6709\u83ab\u5927\u7684\u52a9\u76ca\u3002<\/p>\n

 <\/p>\n

\u76ee\u524d\u7814\u7a76\u91cd\u9ede\uff1a<\/b><\/p>\n

I.<\/b>\u751f\u7269\u89f8\u5a92\u5316\u5b78\uff1a\u6838\u9178\u9176(<\/b>ribozyme<\/b>)<\/b>\u7684\u4eba\u70ba\u6f14\u5316\u7be9\u9078\u3001\u7d50\u69cb\u3001\u52d5\u529b\u5b78\u7279\u6027\u3001\u53ca\u50ac\u5316\u53cd\u61c9\u6a5f\u5236\u5206\u6790\u63a2\u8a0e<\/b><\/p>\n

\u672c\u7814\u7a76\u4e3b\u984c\u662f\u7d93\u7531\u4eba\u70ba\u6f14\u5316(in vitro<\/i> evolution)\u7684\u65b9\u6cd5\uff0c\u4ee5\u7d44\u5408\u5316\u5b78(combinatory chemistry)\u65b9\u8a66\u6240\u7be9\u9078\u51fa\u5177\u6709\u50ac\u5316\u529f\u80fd\u7684\u6838\u91a3\u6838\u9178\u5206\u5b50(RNA)\uff0c\u53c8\u7a31\u70ba\u6838\u9178\u9176\u3002\u9019\u4e9b\u7be9\u9078\u51fa\u7684\u6838\u9178\u9176\u500b\u5206\u5b50\uff0c\u7d93\u7531\u50ac\u5316\u53cd\u61c9\u52d5\u529b\u5b78\u7684\u901f\u7387\u5e38\u6578\u6e2c\u91cf\u5206\u6790\uff0c\u6211\u5011\u53ef\u4ee5\u4e86\u89e3\u6838\u9178\u9176\u7684\u53d7\u8cea\u5c08\u4e00\u6027\u3001\u4e00\u7d1a\u53ca\u4e8c\u7d1a\u7d50\u69cb\u3001\u53d7\u8cea\u6216\u91d1\u5c6c\u96e2\u5b50\u7d50\u5408\u4f4d\u7f6e\u4e4b\u5340\u57df\u7d50\u69cb\u3001\u91d1\u5c6c\u96e2\u5b50\u76f8\u4f9d\u6027\u53caRNA\u5167\u91d1\u5c6c\u96e2\u5b50\u87af\u5408\u7684\u578b\u614b\u3001\u9178\u9e7c\u5c0d\u50ac\u5316\u4e4b\u5f71\u97ff\u3001\u53ca\u671f\u50ac\u5316\u53cd\u61c9\u6a5f\u5236\u63a8\u6f14\u3002\u9019\u4e9b\u7d50\u679c\u53ef\u4ee5\u5e6b\u52a9\u6211\u5011\u5efa\u7acb\u985e\u4f3c\u86cb\u767d\u8cea\u9175\u7d20\u50ac\u5316\u7684in trans<\/i>\u7cfb\u7d71\u3002\u672c\u7814\u7a76\u7684\u6838\u5fc3\u76ee\u6a19\uff0c\u5728\u65bc\u6df1\u5165\u63a2\u8a0e\u6838\u9178\u9176\u7684\u50ac\u5316\u6a5f\u5236\uff0c\u4e86\u89e3\u5176\u57fa\u672c\u7684\u50ac\u5316\u539f\u7406\uff0c\u4e26\u8207\u985e\u4f3c\u50ac\u5316 \u529f\u80fd\u7684\u86cb\u767d\u8cea\u9175\u7d20\u6bd4\u8f03\uff0c\u4e86\u89e3\u9019\u4e9b\u751f\u7269\u50ac\u5316\u5291\u50ac\u5316\u6a5f\u7406\u7684\u7570\u540c\u3002\u672a\u4f86\u6211\u5011\u5c07\u6703\u6301\u7e8c\u7d50\u5408\u6709\u6a5f\u5408\u6210\u53ca\u7d44\u5408\u5316\u5b78\u7684\u65b9\u5f0f\uff0c\u5229\u7528\u4eba\u70ba\u6f14\u5316\uff0c\u958b\u767c\u66f4\u591a\u5177\u6709\u50ac\u5316\u7279\u6b8a\u5316\u5b78\u53cd\u61c9 \u7684\u6838\u9178\u9176\u3001\u63a2\u8a0e\u5b83\u5011\u7684\u7d50\u69cb\u3001\u50ac\u5316\u7279\u6027\u3001\u53cd\u61c9\u6a5f\u5236\u3001\u53ca\u5176\u751f\u7269\u79d1\u6280\u61c9\u7528\u3002\u6211\u5011\u4e2d\u9060\u7a0b\u7814\u7a76\u76ee\u6a19\uff0c\u662f\u5229\u7528\u6838\u9178\u9176\u7684\u50ac\u5316\u6d3b\u6027\u4f86\u767c\u5c55\u751f\u7269\u611f\u6e2c\u5668\u3002<\/p>\n

 <\/p>\n

II.<\/b>\u6838\u9178\u5316\u5b78\u53ca\u6838\u9178\u85e5\u7269\u4e4b\u7814\u7a76\u958b\u767c\uff1b<\/b>phosphormaidation<\/b>\u53cd\u61c9\u4e4b\u61c9\u7528\u65bc\u6838\u9178\u5206\u5b50\u6a19\u5b9a\u4fee\u98fe\u53ca<\/b>POCs<\/b>\u7684\u5408\u6210\u5099\u88fd<\/b><\/p>\n

\u672c\u7814\u7a76\u690d\u57fa\u65bc\u6211\u5011\u5be6\u9a57\u5ba4\u5148\u524d\u958b\u767c\u51fa\u7684\u6838\u9178phosphoramidation\u53cd\u61c9\u65b9\u6cd5\uff0c\u63d0\u4f9b\u91cd\u8981\u7684\u6838\u9178\u7d50\u69cb\u4fee\u98fe\u5e73\u53f0\uff0c\u5f97\u4ee5\u6709\u6548\u7684\u5c07\u5c0f\u5206\u5b50\u4f8b\u5982biotin\u985e\u5206\u5b50\u6216fluorescein\u7b49\u87a2\u5149\u5206\u5b50\uff0c\u6216\u662f\u4f8b\u5982\u80dc\u80bd\u3001\u86cb\u767d\u8cea\u7b49\u5927\u578b\u751f\u7269\u5206\u5b50\uff0c\u5171\u50f9\u9375\u7d50\u4e0aDNA\u6216RNA\uff0c\u6210\u529f\u89e3\u6c7a\u76ee\u524d\u6838\u9178conjugation\u53cd\u61c9\u7684\u4f4e\u7522\u7387\u4f4e\u6548\u80fd\u554f\u984c\uff1b\u672c\u5be6\u9a57\u5ba4\u76ee\u524d\u66f4\u5c07\u6838\u9178phosphoramidation\u53cd\u61c9\uff0c\u8207click chemistry\u53caStaudinger ligation\u7b49\u91cd\u8981bioorthogonal\u53cd\u61c9\u7d50\u5408\uff0c\u767c\u5c55\u66f4\u9ad8\u6548\u80fd\u7684\u6838\u9178conjugation\u53cd\u61c9\uff0c\u505a\u70ba\u672a\u4f86\u958b\u767c\u5ee3\u7528\u6027\u6838\u9178\u5206\u5b50\u6a19\u5b9a\u65b9\u6cd5\u53ca\u6838\u9178\u751f\u7269\u611f\u61c9\u5668\u4e4b\u57fa\u790e\u3002\u672c\u5be6\u9a57\u5ba4\u4e5f\u5df2\u6210\u529f\u5229\u7528\u8a72\u65b9\u6cd5\uff0c\u6709\u6548\u7684\u5099\u88fd\u51fapeptide-oligonucleotide conjugates (POCs)\uff0c\u7d93\u7531\u61c9\u7528\u751f\u7269\u9ad4\u5167\u7684antisense DNA\u4e4bgene silencing\uff0c\u6216\u662finterference RNA\u53camicroRNA\u4e4b\u4fc3\u9032mRNA\u751f\u7269\u964d\u89e3\u7b49\u6a5f\u5236\uff0c\u672a\u4f86\u61c9\u7528\u65bc\u81e8\u5e8a\u6cbb\u7642\u53ca\u57fa\u790e\u7814\u7a76\uff0c\u7814\u767c\u51fa\u66f4\u5177\u5c08\u4e00\u6027\u4e14\u7121\u526f\u4f5c\u7528\u7684\u6838\u9178\u85e5\u7269\uff0c\u9054\u5230\u75be\u75c5\u6cbb\u7642\u7684\u76ee\u6a19\u3002\u7d93\u7531\u6211\u5011\u5c0d\u6838\u9178phosphoramidation\u53cd\u61c9\u7684\u57fa\u790e\u6709\u6a5f\u53cd\u61c9\u6a5f\u7406\u63a2\u8a0e\uff0c\u6539\u5584phosphoramidation\u53cd\u61c9\u65b9\u6cd5\uff0c\u5df2\u7d93\u986f\u8457\u7684\u63d0\u5347POCs\u7684\u7522\u7387\uff0c\u589e\u52a0\u9019\u500b\u53cd\u61c9\u7684\u5be6\u7528\u53ca\u5ee3\u7528\u6027\uff1b\u6211\u5011\u5c07\u6301\u7e8c\u52aa\u529b\uff0c\u8b93phosphoramidation\u53cd\u61c9\u6210\u70ba\u672a\u4f86\u5099\u88fdPOCs\u7684\u4e3b\u6d41\u65b9\u6cd5\u3002\u6211\u5011\u5c07\u6703\u4ee5\u5927\u8178\u687f\u83cc(E. coli<\/i>)\u4f86\u7576\u4f5c\u6211\u5011\u7684\u6a21\u578b\u7cfb\u7d71\uff0c\u8b49\u660e\u6211\u5011\u6240\u8a2d\u8a08\u7684POCs\u53ef\u4ee5\u6291\u5236\u7d30\u83cc\u7684\u751f\u9577\uff0c\u9032\u800c\u8b49\u5be6POCs\u5728\u672a\u4f86\u6240\u6709\u75be\u75c5\u6cbb\u7642\u4e0a\u7684\u6f5b\u529b\u3002\u5e0c\u671b\u518d\u672a\u4f86\u6211\u5011\u5c07\u80fd\u5920\u4ee5\u6838\u9178\u8f14\u52a9\u5c0f\u5206\u5b50\u85e5\u7269\uff0c\u80fd\u66f4\u6709\u6548\u7684\u6cbb\u7642\u6240\u6709\u75be\u75c5\uff0c\u964d\u4f4e\u75c5\u4eba\u56e0\u670d\u7528\u85e5\u7269\u7684\u526f\u4f5c\u7528\uff0c\u589e\u9032\u5168\u4eba\u985e\u7684\u5065\u5eb7\u798f\u7949\u3002<\/p>\n

 <\/p>\n

III.\u87a2\u5149\u5316\u5b78\u63a2\u91dd(chemical probes)\u4e4b\u6709\u6a5f\u5408\u6210:\u5229\u7528\u751f\u7269\u6b63\u4ea4(bioorthogonal)\u53cd\u61c9\uff0c\u65bc\u6709\u6a5f\u5408\u6210\u87a2\u5149\u5c0f\u5206\u5b50\u63a2\u91dd\uff0c\u4ee5\u70ba\u9175\u7d20\u6d3b\u6027\u6216\u65e9\u671f\u75be\u75c5\u767c\u751f\u6aa2\u6e2c\u4e4b\u8a66\u5291\u3002<\/strong><\/p>\n

\u6b64\u70ba\u672c\u5be6\u9a57\u5ba4\u76ee\u524d\u4e3b\u8981\u7814\u7a76\u91cd\u9ede\u3002\u5728\u672c\u7814\u7a76\u4e2d\uff0c\u6211\u5011\u5408\u6210\u51fa\u95dc\u9375\u7684bicyclononyne (BCN)\u884d\u751f\u7269\uff0c\u4e26\u5229\u7528copper-free\u76841,3-dipolar azide-alkyne cycloaddition\u53cd\u61c9\uff0c\u5c07\u87a2\u5149\u6216\u5177\u6a19\u9776\u7279\u6027\u5206\u5b50\u4fee\u98fe\u9375\u7d50\u4e0a\uff0c\u7372\u5f97\u5316\u5b78\u63a2\u91dd\uff0c\u4ee5\u505a\u70ba\u80fd\u5b9a\u91cf\u4f8b\u5982butyrylcholinesterase\u7b49\u9175\u7d20\u6d3b\u6027\uff0c\u6216\u662f\u65e9\u671f\u6e2c\u767c\u73fe\u4f8b\u5982\u764c\u7b49\u75c7\u75c5\u4e4b\u8a66\u5291\uff0c\u9054\u5230\u5373\u6642\u75be\u75c5\u6cbb\u7642\u4e4b\u76ee\u6a19\u3002\u56e0\u6b64\u672c\u7814\u7a76\u4ee5\u6709\u6a5f\u5408\u6210\u70ba\u4e3b\uff0c\u914d\u5408\u87a2\u5149\u5149\u8b5c\u5b78\uff0c\u9175\u7d20\u52d5\u529b\u5b78\u3001\u53ca\u7d30\u80de\u751f\u7269\u87a2\u5149\u5f71\u50cf\uff0c\u5efa\u69cb\u51fa\u4ee5\u5316\u5b78\u70ba\u4e3b\u9ad4\u7684\u8de8\u9818\u57df\u7814\u7a76\u3002<\/p>\n

 <\/p>\n

IV.<\/b>\u74ca\u916f\u91a3<\/b>(Agarose)<\/b>\u5316\u5b78\uff1a\u751f\u91ab\u5206\u751f\u7d1a\u7684\u74ca\u916f\u91a3\u4e4b\u9187\u5316\u53ca\u5176\u884d\u751f\u7269\u4e4b\u958b\u767c<\/b><\/p>\n

\u672c\u7814\u7a76\u4e3b\u984c\u662f\u7d93\u7531\u570b\u7522\u7d05\u85fb\u985e\u690d\u7269\uff0c\u7d14\u5316\u51fa\u80fd\u61c9\u7528\u65bc\u751f\u91ab\u5206\u751f\u7814\u7a76\u7b49\u7d1a\u7684\u9ad8\u7d14\u5ea6\u4f4e\u80cc\u666f\u503c\u7684\u74ca\u916f\u91a3\uff0c\u518d\u85c9\u8457\u7269\u7406\u6216\u5316\u5b78\u8f49\u63db\u6216\u4fee\u98fe\uff0c\u751f\u7522\u51fa\u66f4\u9ad8\u55ae\u50f9\u7684\u4f4e\u7194\u9ede\u74ca\u916f\u91a3\u3002\u9019\u4e9b\u7d14\u5316\u51fa\u4f86\u7684\u74ca\u916f\u91a3\uff0c\u53ef\u4ee5\u7d93\u7531\u7269\u7406\u6216\u5316\u5b78\u8655\u7406\uff0c\u751f\u7522\u51fa\u74ca\u916f\u91a3\u81a0\u9ad4\u7c92\u5b50(agarose bead)\u3001\u9032\u800c\u7814\u767c\u8655\u53ef\u61c9\u7528\u65bc\u751f\u7269\u5206\u5b50\u7d14\u5316\u5206\u96e2\u4e14\u66f4\u9ad8\u7d93\u6fdf\u50f9\u503c\u7684\u74ca\u916f\u91a3\u81a0\u9ad4\u7c92\u5b50\u884d\u751f\u7269\uff0c\u4e26\u4e14\u4e5f\u53ef\u7528\u65bc\u958b\u767c\u591a\u5b54\u6027\u7684\u7d44\u7e54\u518d\u751f\u7684\u57fa\u8cea\u6750\u6599\u3002\u672c\u7814\u7a76\u6210\u679c\u4e0d\u53ea\u53ef\u4ee5\u6eff\u8db3\u570b\u5167\u76ee\u524d\u751f\u91ab\u5206\u751f\u7814\u7a76\u6240\u9700\u6c42\u7684\u74ca\u916f\u81a0\u53ca\u5176\u884d\u751f\u7269\uff0c\u4e5f\u80fd\u5c07\u9019\u4e9b\u7522\u54c1\u5916\u92b7\u570b\u5916\u3001\u63d0\u5347\u53f0\u7063\u7d93\u8cbf\u5be6\u529b\u53ca\u751f\u6280\u7522\u696d\u672a\u4f86\u767c\u5c55\u7684\u7a7a\u9593\uff1b\u6700\u5f8c\u4e5f\u53ef\u85c9\u7531\u63d0\u5347\u672c\u571f\u7d05\u85fb\u985e\u690d\u7269\u7684\u7d93\u6fdf\u6548\u76ca\uff0c\u6539\u5584\u6cbf\u6d77\u5c45\u6c11\u7684\u7d93\u6fdf\u80fd\u529b\u6975\u4fc3\u9032\u570b\u571f\u7684\u6c38\u7e8c\u958b\u767c\u5229\u7528\u3002<\/p>\n

 <\/p>\n

\u672a\u4f86\u8a08\u756b\u5ef6\u4f38\u7814\u7a76\u4e3b\u984c\uff1a<\/b><\/p>\n

\u751f\u7269\u7121\u6a5f\u5316\u5b78\uff1a\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u8207\u8166\u5167\u91d1\u5c6c\u96e2\u5b50\u5e73\u8861\u5931\u8abf\u53ca\u5176\u6cbb\u7642<\/b><\/p>\n

\u6211\u5011\u5be6\u9a57\u5ba4\u5728\u672a\u4f86\uff0c\u5e0c\u671b\u80fd\u9032\u884c\u9ad8\u7b49\u751f\u7269\u7684\u91d1\u5c6c\u65b0\u9673\u4ee3\u8b1d\u7684\u57fa\u790e\u7814\u7a76\uff0c\u7279\u5225\u6ce8\u91cd\u65bc\u7814\u7a76\u91d1\u5c6c\u96e2\u5b50\u65bc\u54fa\u4e73\u985e\u52d5\u7269\u8166\u90e8\u7684\u8abf\u63a7\u5e73\u8861(homesstasis imbalance)\u8207\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u4f8b\u5982\u5e15\u91d1\u68ee\u6c0f\u75c7(Parkinson\u2019s disease)\u3001\u963f\u8332\u6d77\u9ed8\u6c0f\u75c7(Alzheimer\u2019s disease)\u3001\u53caprion diseases\u7b49\u4e4b\u56e0\u679c\u95dc\u4fc2\uff0c\u9032\u800c\u7531\u7121\u6a5f\u751f\u7269\u5316\u5b78\u7684\u89d2\u5ea6\uff0c\u8a2d\u8a08\u5408\u6210\u9069\u7576\u7684\u85e5\u7269\uff0c\u505a\u70ba\u5728\u672a\u4f86\u80fd\u6709\u6548\u7684\u7de9\u5408\u6216\u6cbb\u7642\u9019\u4e9b\u75be\u75c5\uff0c\u6e1b\u8f15\u75c5\u4eba\u7684\u75db\u82e6\u53ca\u5bb6\u4eba\u7684\u8ca0\u64d4\uff1b\u6211\u5011\u4e5f\u5e0c\u671b\u80fd\u627e\u51fa\u9069\u7576\u975e\u4fb5\u5165\u6027\u7684\u5206\u6790\u65b9\u6cd5\uff0c\u9019\u4e9b\u75be\u75c5\u7684\u767c\u5c55\u65e9\u671f\uff0c\u5c31\u80fd\u4e88\u4ee5\u6aa2\u6e2c\u4e26\u6709\u6548\u7684\u6cbb\u7642\uff0c\u9632\u6b62\u9019\u4e9b\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u7684\u60e1\u5316\u53ca\u7522\u751f\u3002<\/p>\n

 <\/p>\n

\u5c08\u5229\u767c\u660e<\/strong><\/p>\n

    \n
  1. Wang, T.-P. (\u738b\u5b50\u658c),<\/strong> C.-Y. Dai (\u6234\u5609\u8a00) , B.-K. Fang (\u65b9\u67cf\u51f1), and C.-H. Huang (\u9ec3\u828a\u60e0) (2023<\/strong>) \u96d9\u74b0\u58ec\u7094\u884d\u751f\u7269\u53ca\u5176\u88fd\u5099\u65b9\u6cd5\u548c\u7528\u9014(Bicyclononyne derivatives and manufacturing method and use thereof), Taiwan Patent No.<\/em>: I818431<\/strong>, Date of Patent: Oct 11, 2023.<\/li>\n
  2. Wang, T.-P.<\/strong> (\u738b\u5b50\u658c<\/strong><\/strong>)and Y.-C. Su (\u8607\u90c1\u667a) (2018<\/strong>) Reagents for universal site-specific labeling and modifications of nucleic acids, U.S. Patent No.: US 9,856,285 B2<\/strong>, Date of Patent: Jan. 02, 2018.<\/li>\n
  3. Wang, T.-P.<\/strong> (\u738b\u5b50\u658c<\/strong><\/strong>), N. C. Ko (\u67ef\u9713\u84a8), and Y.-C. Su (\u8607\u90c1\u667a) (2015<\/strong>)\u6838\u9178\u5171\u8edb\u7269\u7684\u6db2\u76f8\u5408\u6210\u6cd5(Liquid phase synthesis of a nucleic acid conjugate), Taiwan Patent No.<\/em>: I471331<\/strong>, Date of Patent: Feb. 1, 2015.<\/li>\n
  4. Wang, T.-P.\u00a0<\/strong>(\u738b\u5b50\u658c<\/strong><\/strong>) and Y.-J. Chiou (\u90b1\u5955\u748b) (2014<\/strong>)\u5c07\u6838\u9178\u8857\u4e0a\u5c0f\u5206\u5b50\u7684\u65b9\u6cd5(Methods for conjugating nucleic acids with small molecules), Taiwan Patent No.<\/em>: I464402<\/strong>, Date of Patent: Dec. 11, 2014.<\/li>\n
  5. Wang, T.-P.<\/strong> (\u738b\u5b50\u658c<\/strong>) and Y.-J. Chiou (\u90b1\u5955\u748b) (2014<\/strong>) Methods for conjugating nucleic acids with small molecules, U.S. Patent No.<\/em>: US 8,765,934 B2<\/strong>, Date of Patent: Jul. 1, 2014.<\/li>\n<\/ol>\n

     <\/p>\n

    \u904e\u53bb\u5341\u5e74\u671f\u520a\u8457\u4f5c<\/strong><\/p>\n

    1.<\/strong> Huang, C.-H., C.-Y. Dai, S.-H. Wang, S. Severance, C.-C. Hwang, Y.-C. Liu, B.-L. Yeh, Y.-C. Weng, S.-L. Yu, H.-T. Kuo, L.-F. Wang, J.-J. Wang, and T.-P. Wang*<\/strong> (2025<\/strong>) Harnessing contact-quenched, profluorescent chemical probes for sensitive determination and high-throughput measurements of enzyme activity. ACS Omega<\/em>, 10 <\/strong>(48<\/strong>), 58566\u221258576.<\/p>\n

    2.<\/strong> Mutra, M. R., T. L. Chandana, T.-P. Wang<\/strong>, and J.-J. Wang*<\/sup> (2025<\/strong>) Ag(I)\/K2<\/sub>S2<\/sub>O8<\/sub>\u2011<\/strong>mediated selective oxidation of ynamide-yne via structural reshuffling and consecutive N<\/em>\u2011<\/strong>desulfonylation. J. Org. Chem.<\/em>, 90<\/strong>, 10031\u221210036.<\/p>\n

    3. <\/strong>Huang, C.-H., S.-Y. Hou, S. Severance, C.-C. Hwang, B.-K. Fang, M.-M. Gong, S.-L. Yu, Y.-C. Weng, L.-F. Wang, C.-Y. Dai, S.-H. Wang, H.-T. Kuo, J.-J. Wang, and T.-P. Wang<\/strong>* (2023<\/strong>) Manipulating diastereomeric bicyclononynes to sensitively determine enzyme activity and facilitate macromolecule conjugations. ACS Omega<\/em>, 8<\/strong> (48<\/strong>), 46073\u221246090. http:\/\/doi.org\/10.1021\/acsomega.3c07083.<\/p>\n

    4. <\/strong>Chou, Y.-H., C.-L. Hsieh, Y.-L. Chen, T.-P. Wang<\/strong>, W.-J. Wu, C.-C. Hwang*<\/sup> (2023<\/strong>) Characterization of the pH-dependent protein stability of 3\u03b1-hydroxysteroid dehydrogenase\/carbonyl reductase by differential scanning fluorimetry. Protein Sci., <\/em>32<\/strong> (8<\/strong>), e4710. https:\/\/doi.org\/10.1002\/pro.4710<\/p>\n

    5.<\/strong> Chen,Y.-L., Y.-H. Chou, C.-L. Hsieh, S.-J. Chiou, T.-P. Wang<\/strong> and Chi-Ching Hwang*<\/sup>. (2022<\/strong>) Rational Engineering of 3a-hydroxysteroid dehydrogenase\/carbonyl reductase for a biomimetic nicotinamide mononucleotide cofactor. Catalysts<\/em>, 12 (10)<\/strong>, 1094. doi: 10.3390\/catal12101094.<\/p>\n

    6.<\/strong> Huang, C.-H., Y.-T. Chang, S. Severance, J.-Y. Feng S.-Y. Hou, M.-M. Gong, C.-C. Hwang, C.\u2010Y. Dai, J.-J. Wang and T.-P. Wang*<\/strong>. (2022<\/strong>) Time-dependent effects of storage at \u201380 \u00b0C on the stability of butyrylcholinesterase activity in human serum. Pract. Lab. Med.<\/em>, 31<\/strong>, e00298. https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2352551722000373<\/p>\n

    7. <\/strong>Fang, B.-K., C.-Y. Dai, S. Severance, C.-C. Hwang, C.-H. Huang, S.-Y. Hou, B.-L. Yeh, M.-M. Gong , Y.-H. Chou, J.-J. Wang and T.-P. Wang*<\/strong>. (2022<\/strong>) Sensitive assay for the lactonase activity of serum paraoxonase 1 (PON1) by harnessing the fluorescence turn-on characteristics of bioorthogonally synthesized and geometrically controlled chemical probes. Molecules<\/em>, 27<\/strong> (8<\/strong>), 2435. https:\/\/www.mdpi.com\/1420-3049\/27\/8\/2435\/htm<\/p>\n

    8.<\/strong> Gong, M.-M., C.-Y. Dai, S. Severance, C.-C. Hwang, B.-K. Fang, H.-B. Lin, C.-H. Huang, C.-W. Ong, J.-J. Wang, P.-L. Lee and T.-P. Wang*<\/strong>. (2020<\/strong>) Bioorthogonally synthesized and disulfide-containing fluorescence turn-on chemical probe for measurements of butyrylcholinesterase activity and inhibition in the presence of physiological glutathione. Catalysts<\/em>, 10 (10)<\/strong>, 1169. doi:10.3390\/catal10101169<\/p>\n

    9.<\/strong> Hwang, C.-C.,*<\/sup> P.-R. Chang, C.-L. Hsieh, Y.-H. Chou, T.-P. Wang<\/strong>. (2019<\/strong>) Thermodynamic analysis of remote substrate binding energy in 3\u03b1-hydroxysteroid dehydrogenase\/carbonyl reductase catalysis. Chem. Biol. Interact.<\/em>, 302<\/strong>, 183-189.<\/p>\n

    10. Wang, T.-P. *<\/strong>, Y.-C. Su, Y. Chen, S. Severance, C.-C. Hwang, Y.-M. Liou, C.-H. Lu, K.-L. Lin, R. J. Zhu, E.-C. Wang. (2018<\/strong>) Corroboration of Zn(II)-Mg(II)-tertiary structure interplays essential to optimal catalysis of a phosphorothiolate thiolesterase ribozyme. RSC Adv.<\/em>, 8<\/strong>, 32775-32793. https:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2018\/ra\/c8ra05083j<\/p>\n

    11. <\/strong>Hwang, C.-C.*, P.-R. Chang, T.-P. Wang<\/strong>. (2017<\/strong>) Contribution of remote substrate binding energy to the enzymatic rate acceleration for 3\u03b1-hydroxysteroid dehydrogenase\/carbonyl reductase. Chem. Biol. Interact.<\/em> , 276<\/strong>, 133-140.<\/p>\n","protected":false},"excerpt":{"rendered":"

    \u00a0Here for English \u00a0 \u00a0 \u8fa6\u516c\u5ba4\uff1aN817\u5be6\u9a57\u5ba4(N835\u5f8c\u9762 … \u95b1\u8b80\u5168\u6587 →<\/span><\/a><\/p>\n","protected":false},"author":12,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"onecolumn-page.php","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"class_list":["post-2","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wp.kmu.edu.tw\/tzupinw\/wp-json\/wp\/v2\/pages\/2","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wp.kmu.edu.tw\/tzupinw\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wp.kmu.edu.tw\/tzupinw\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wp.kmu.edu.tw\/tzupinw\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/wp.kmu.edu.tw\/tzupinw\/wp-json\/wp\/v2\/comments?post=2"}],"version-history":[{"count":28,"href":"https:\/\/wp.kmu.edu.tw\/tzupinw\/wp-json\/wp\/v2\/pages\/2\/revisions"}],"predecessor-version":[{"id":97,"href":"https:\/\/wp.kmu.edu.tw\/tzupinw\/wp-json\/wp\/v2\/pages\/2\/revisions\/97"}],"wp:attachment":[{"href":"https:\/\/wp.kmu.edu.tw\/tzupinw\/wp-json\/wp\/v2\/media?parent=2"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}