Jack Hsu Lab.

    Cancer,  medically known as a malignant neoplasm, is a broad group of various diseases and directly affects at least one-third of the human population, all involving unregulated cell growth. In cancer, cells divide and grow uncontrollably, forming malignant tumors, and invade nearby parts of the bodyby EMT. The cancer may also spread to more distant parts of the body through the lymphatic system or bloodstream. Not all tumors are cancerous. Benign tumors do not grow uncontrollably, do not invade neighboring tissues, and do not spread throughout the body. There are over 200 different known cancers that afflict humans.[1]

   Despite this extensive research prevalence, the genetic determinants of cancer risk remain largely unknown. Highly potent mutations of proto- oncogenes as well as tumor suppressor genes, and more subtle and complex genetic interactions within each individual are currently thought to influence susceptibility to cancer


Hepatocellular Carcinoma and leukemia

In our laboratory, our long-term goal is to define the relationship between genes, development, and cancers through molecular and cell biology coupling clinical evidences application. From 2005 to 2012, several novel genes were cloned for further characterization through the molecular cloning approach. The pathogenic functions in cancer development were identified to ensure whether it was a proto-oncogene, including GPR177(WLS), SPZ1, and ISX. In the secondary exploration stage from 2012–2017, extension studies were addressed on the molecular regulation mechanism, spectrums of downstream target, and potential oncogenic activities of these proto-oncogenes in cancer cells transcriptionally. Currently (2017–2022), our study aimed to clarify the pathogenic crosstalk effects of these proto-oncogenes on tumor microenvironment editing in both immune and cancer cells when cancer metastasis occurs. During the past 5 years, the underlying proto-oncogenes regulatory mechanism in the enhancement of tumor epithelial-to-mesenchymal transition (EMT) were characterized and verified. The regulatory roles of immune cells, such as dendritic cells (DCs) and macrophages (MΦ), in tumor EMT and microenvironment editing were addressed and verified. These proto-oncogenes, like a double sword, showed differential regulatory effects on both immune and cancer cells contributing different effects on cancer metastasis. The relevant regulatory effects of WLS, SPZ1, and ISX in vitro and in vivo were characterized in our laboratory. Some have been published in several important cell biology and oncology journals (Autophagy, Cancer Letters, Oncogene and Cancer Research).

Shih-Hsien, Hsu Ph. D./Professor

(許世賢 博士/教授)

No100, Shi-Chuan 1st Road, San Ming District,807 Kaohsiung , Taiwan, R.O.C.
Tel:886-7-23121101 Ext 2136#28 Fax:886-7-3118902
E-mail: jackhsu@kmu.edu.tw

Position:  Professor, Graduate Institute of Medicine, Kaohsiung Medical University (KMU)

Present Job:

  • 2018-Now Director of Master’s program, Graduate Institute of Medicine, Kaohsiung Medical University (KMU).
  • 2017-Now Professor, Graduate Institute of Medicine, Kaohsiung Medical University (KMU).
  • 2019-Now Voice CEO, Center of Applied Genomics, Kaohsiung Medical University (KMU)


Ph. D.: 1996-2001 National Defense Medical Center, Taipei, Taiwan.                     (Laboratory: Dr. Hung Li (Institute of Molecular Biology, Academia Sinica.)


  1. Development Biology
  2. Gene transcription and Regulation
  3. Molecular oncology
  4. Immunotherapy

Referees of expertise journal :

  1. Hepatology
  2. Oncogene
  3. Cell Death & Disease
  4. Molecular therapy
  5. Cancers
  6. Biomedicines


  1. Pharmaceutical composition of regulating the expression level of survival of motor neuron 1 and method of detecting enzyme activity of ubiquitin carboxyl-terminal hrdrolase L1 in human fibroblasts TW:100104844; US:13/032846.
  2. Method of regulating the expression level of survival of motor neuron 1. US 9,045,793B2. (06.09)
  3. 104015-TW, 105T-11-052-080.

Awards and Honors:

  1. 2000. Excellent Poster Award, 8th Symposium on Recent Advences in Cellular and Molecular Biology.
  2. 2001. Excellent Poster Award, 9th Symposium on Recent Advences in Cellular and Molecular Biology.
  3. 2007.Excellent Poster Award, 15th Symposium on Recent Advences in Cellular and Molecular Biology.
  4. 2010. Excellent Poster Award, 25th Symposium on Recent Advences in Cellular and Molecular Biology.
  5. 2011. Outstanding Research award, Kaohsiung Medical University.
  6. 2013-2014. Travel Grant award, 72nd -73rd Annual Meeting of The Japanese Cancer Association (JCA).
  7. 2013-2014. Outstanding Research award, Kaohsiung Medical University
  8. 2014 Liver Disease Prevention Research Foundation Fellowship.
  9. 2018-2021. Outstanding Research award, Kaohsiung Medical University.
  10. Annual excellent research project funding, Kaohsiung Medical University.
  11. 2018, 2120-2022 Special Outstanding Talents of the Ministry of Science and Technology Awards, Ministry of Science and Technology, R.O.C., Taiwan.
  12. 2022, Tung Ta-Cheng’s Basic Cancer Research Award.


  1. Wang LT, Liu KY, Wang SN, Lin MH, Liao YM, Lin PC, Huang SK,Shih-Hsien Hsu*, Chiou SS*. Aryl hydrocarbon receptor-kynurenine axis promotes oncogenic activity in BCP-ALL. Cell Biol Toxicol. 2022 Jun 10. doi: 10.1007/s10565-022-09734-0. Online ahead of print.
  2. Li-Ting Wang, Kwei-Yan Liu , Shyh-Shin Chiou, Shau-Ku Huang,Shih-Hsien Hsu*, Shen-Nien Wang*. Phosphorylation of intestine-specific homeobox by ERK1 modulates oncogenic activity and sorafenib resistance. Cancer letters. 2021 Jul 12; 520: 160-171.
  3. Chuang KT, Wang SN,Shih-Hsien Hsu, Wang LT. Impact of bromodomain-containing protein 4 (BRD4) and intestine-specific homeobox (ISX) expression on the prognosis of patients with hepatocellular carcinoma’ for better clarity. Cancer Med. 2021 Aug;10(16):5545-5556.
  4. Li-Ting Wang#, Ming-Hong Lin#, Kwei-Yan Liu, Shyh-Shin Chiou, Shen-Nien Wang, Chee-Yin Chai, Li-Wen Tseng, Hsin-Ying Clair Chiou, Hsueh-Chun Wang, Kazunari K. Yokoyama, Shih-Hsien Hsu*, Shau-Ku Huang*. WLS/wntless is essential in controlling dendritic cell homeostasis via a WNT signaling-independent mechanism. Autophagy. 2021 Dec;17(12):4202-4217.
  5. Kwei-Yan Liu, Li-Ting Wang, Hsueh-Chun Wang, Shen-Nien Wang, Li-Wen Tseng, Chee-Yin Chai, Shyh-Shin Chiou, Shau-Ku Huang*, Shih-Hsien Hsu*. Aryl Hydrocarbon Receptor is Essential in the Control of Lung Club Cell Homeostasis. 2021 Jan. Journal of Inflammation Research 2021 Feb 5;14:299-311.
  6. Wang LT, Liu KY, Jeng WY, Chiang CM, Chai CY, Chiou SS, Huang MS, Yokoyama KK, Wang SN, Huang SK, Shih-Hsien Hsu*. PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transition. EMBO Rep. 2020 Feb 5;21(2):e48795. Highlighted by scrolling marquee of EMBO Reports.
  7. Liu KY, Wang LT, Shih-Hsien Hsu*, Wang SN*. Homeobox Genes and Hepatocellular Carcinoma. Cancers (Basel). 2019 May 3;11(5). pii: E621. (Invited review)
  8. Wang LT, Wang SN, Chiou SS, Liu KY, Chai CY, Chiang CM, Huang SK, Yokoyama KK, Shih-Hsien Hsu*. TIP60-dependent acetylation of the SPZ1-TWIST complex promotes epithelial-mesenchymal transition and metastasis in liver cancer. Oncogene. 2019 Jan;38(4):518-532. doi: 10.1038/s41388-018-0457-z. [Epub ahead of print]
  9. Chiu YH#,  Shih-Hsien Hsu#, Hsu HW, Huang KC, Liu W, Wu CY, Huang WP, Chen JY, Chen BH, Chiu CC. Human non‑small cell lung cancer cells can be sensitized to camptothecin by modulating autophagy. Int J Oncol. 2018 Nov;53(5):1967-1979. (# equal contribute to this manuscript)
  10. Wang HC, Wong TH, Wang LT, Su HH, Yu HY, Wu AH, Lin YC, Chen HL, Suen JL, Hsu SH, Chen LC, Zhou Y, Huang SK. Aryl hydrocarbon receptor signaling promotes ORMDL3-dependent generation of sphingosine-1-phosphate by inhibiting sphingosine-1-phosphate lyase. Cell Mol Immunol. 2018 Mar 23. doi: 10.1038/s41423-018-0022-2.
  11. Liu KY, Wang LT, Shih-Hsien Hsu*. Modification of Epigenetic Histone Acetylation in Hepatocellular Carcinoma. Cancers (Basel). 2018 Jan 3;10(1). pii: E8. (Invited review)
  12. Wang YS, Hsi E, Cheng HY, Hsu SH, Liao YC, Juo SH. Let-7g suppresses both canonical and non-canonical NF-κB pathways in macrophages leading to anti-atherosclerosis. 2017 May 23. doi: 10.18632/oncotarget.18197.
  13. Li-Ting Wang, Shyh-Shin Chiou, Chee-Yin Chai, Edward Hsi, Kazunari K. Yokoyama, Shen-Nien Wang, Shau-Ku Huang, Shih-Hsien Hsu*. Intestine-specific homeobox gene ISX integrates interleukin-6 signaling, tryptophan metabolism, and immune suppression. Cancer Research. 2017 Aug 1;77(15):4065-4077.
  14. Shih-Hsien Hsu*, Wang LT, Chai CY, Wu CC, Hsi E, Chiou SS, Wang SN. Aryl hydrocarbon receptor promotes hepatocellular carcinoma tumorigenesis by targeting intestine-specific homeobox expression. Mol Carcinog. 2017 Oct;56(10):2167-2177.
  15. Wang LT, Chiou SS, Chai CY, Hsi E, Chiang CM, Huang SK, Wang SN, Yokoyama KK, Shih-Hsien Hsu*. Transcription factor SPZ1 promotes TWIST-mediated epithelial- mesenchymal transition and oncogenesis in human liver cancer. Oncogene, 2017 Aug;36(31):4405-4414.
  16. Wang LT, Chiou SS, Chai CY, Hsi E, Wang SN, Huang SK, Hsu SH*. Aryl hydrocarbon receptor regulates histone deacetylase 8 expression to repress tumor suppressive activity in hepatocellular carcinoma. Oncotarget. 2017 Jan 31;8(5): 7489-7501.
  17. Chen CY, Yen CY, Wang HR, Yang HP, Tang JY, Huang HW, Hsu SH*, Chang HW*. Tenuifolide B from Cinnamomum tenuifolium Stem Selectively Inhibits Proliferation of Oral Cancer Cells via Apoptosis, ROS Generation, Mitochondrial Depolarization, and DNA Damage. Toxins (Basel). 2016 Nov 5;8(11).
  18. Wang SN, Wang LT, Sun DP, Chai CY, Hsi E, Kuo HT, Yokoyama KK, Hsu SH*. Intestine-specific homeobox (ISX) upregulates E2F1 expression and related oncogenic activities in HCC.Oncotarget.  2016 Jun 14;7(24):36924-36939.
  19. Chen WC, Tseng CK, Chen YH, Lin CK, Hsu SH, Wang SN, Lee JC*. HCV NS5A Up-Regulates COX-2 Expression via IL-8-Mediated Activation of the ERK/JNK MAPK Pathway. PLoS One. 2015 Jul 31;10(7):e0133264.
  20. Shyh-Shin Chiou; Li-Ting Wang; Shih-Bo Huang; Chee-Yin Chai; Shen-Nien Wang; Yu-Mei Liao; Pei-Chin Lin; Kwei-Yan Liu; Shih-Hsien Hsu*. Wntless (GPR177) expression correlates with poor prognosis in B-cell precursor acute lymphoblastic leukemia via Wnt signaling. Carcinogenesis 2014 35 (10):2357-2364.
  21. Li-Ting Wang, Shyh-Shin Chiou, Yu-Mei Liao, Yuh-Jyh Jong, Shih-Hsien Hsu* Survival of motor neuron protein downregulates miR-9 expression in patients with spinal muscular atrophy. Kaohsiung Journal of Medical Sciences 2014 (30), 229-234.


  1. Annual Chinese Conference of male Medical Science.。Testis-Physiology and Pathology, Update.(Keynote Speaker)
  2. Shih-Hsien Hsu and Hung Li, The serum induced novel and leucine zipper contained bHLH transcription factor,Spz1, expressed specially in testes. 1st Development Biology Symposium. (Dan-Shui, Taiwan)
  3. Shih-Hsien Hsuand Hung Li. 2005. Reduced fertility in mice overexpressing bHLH-Zip transcription factor, Spz1. The 9th SCBA International Symposium. (Taipei, Taiwan)
  4. Shih-Hsien Hsuand Yuh-Jyh Jong. 2009. The proteomic approach: UCHL1 regulates SMN protein expression in p19 and SMA fibroblast cells. 25th Annual International Spinal Muscular Atrophy Research Group Meeting June 18-20, (2009 Cincinnati, Ohio)
  5. Li-Ting Wang and Shih-Hsien Hsu. 2010. Functional interaction of Ugene and EBV infection mediates tumorigenic effects. 2nd  Harmonize Gene & Environment : Asian Health Promotion.( Pattaya, Thailand)
  6. Li-Ting Wang and Shih-Hsien Hsu. 2012. Pro-Inflammatory Cytokine-Induced Homeobox Gene, Isx, Regulates Tumorigenic Activity and Survival in Hepatocellular Carcinoma. 22nd Biennial EACR Congress.  Barcelona, Spain, 7-10 July 2012.
  7. Li-Ting Wang and Shih-Hsien Hsu. 2012. Pro-Inflammatory Cytokine-Induced Homeobox Gene, Isx, Regulates Tumorigenic Activity and Survival in Hepatocellular Carcinoma. 71st Annual Meeting of The Japanese Cancer Association (JCA). September 19-21, 2012 in Sapporo, Japan.

在〈Jack Hsu Lab.〉中有 2 則留言

  1. This is a topic which is close to my heart… Many thanks! Exactly where are your contact details though?|